|
| Status |
Public on Nov 10, 2019 |
| Title |
JHOC9 SPINK1 KD #9 replicate a |
| Sample type |
RNA |
| |
|
| Source name |
JHOC9 cells infected with SPINK1 knockdown virus #9 replicate a
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Ovarian clear cell carcinoma
cell line: JHOC9
|
| Treatment protocol |
Effect of gene knockdown
|
| Growth protocol |
JHOC9 cells were maintained in DMEM/F12 (Gibco, Invitrogen) supplemented with 20% fetal bovine serum (FBS) (Gemini Bio-Products, Sacramento, CA, USA). JHOC5 cells were maintained in DMEM/F12 (Gibco, Invitrogen) supplemented with 10% fetal bovine serum (FBS) (Gemini Bio-Products, Sacramento, CA, USA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Phenol/Chloroform
|
| Label |
biotin
|
| Label protocol |
This project used the GeneChip™ Human Transcriptome Array 2.0 200 ng of total RNA was used as input
|
| |
|
| Hybridization protocol |
cRNA was hybridized onto chips for 18 h at 45 degrees C. GeneChips were washed in the Affymetrix Fluidics Station 450 using the EukGE-WS2v4_450 wash protocol.
|
| Scan protocol |
Chips were scanned in an Affymetrix 3000 (7G) Confocal Scanner.
|
| Description |
Gene expression data from cell culture
|
| Data processing |
The data were analyzed with Genespring 14.9.1 (Agilent) using Affymetrix default analysis settings (RMA processing), chips were normalized to 50th percentile, and genes were normalized to average NT values (samples #)
|
| |
|
| Submission date |
Nov 09, 2019 |
| Last update date |
Nov 10, 2019 |
| Contact name |
Derek Charles Radisky |
| E-mail(s) |
radisky.derek@mayo.edu
|
| Phone |
9049536913
|
| Organization name |
Mayo Clinic
|
| Department |
Cancer Biology
|
| Street address |
4500 San Pablo Road
|
| City |
Jacksonville |
| State/province |
Florida |
| ZIP/Postal code |
32224 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE140179 |
Effect of SPINK1 and IL-6 knockdown in JHOC9 and JHOC5 ovarian clear cell carcinoma cells |
|
