Total RNA was extracted from normal and tumor plasma cells using miRNEasy Mini Kit (Qiagen, Valencia, USA) following manufacturer's protocol. The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA).
Label
FAM
Label protocol
Standard Taqman Assay
Hybridization protocol
N/A
Scan protocol
N/A
Description
miR - MM - RB deletion - p891
Data processing
The SDS files are the raw files for real-time PCR data and are readable by Applied Biosystems SDS Software v2.3. SDS Software is available for download from the Applied Biosystems website. Normalization was performed with the mean of RNU44 and RNU48. CT = threshold cycle value dCT = CT_(miRNA) - CT_(control miRNA) for comparisons between MM samples and normal PC. ddCT = dCT_(MM) - dCT_(average normal PC) for comparisons between MM samples. The dCT and ddCT data are presented as log10 of the relative quantity of each miRNA.
