|
| Status |
Public on Sep 04, 2011 |
| Title |
control L606 |
| Sample type |
RNA |
| |
|
| Source name |
non-fasted whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
group: control
gender: female
age: 65.17 years
family: Family 098
hybridization day: Day 09
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Peripheral blood of the 150 non-fasted individuals was harvested using PAXgeneTM tubes (Qiagen). The tubes were frozen and kept at -20°C for ~3-5 years. After thawing at room temperature for at least 2 hours, total RNA was extracted from the approximately 2.5 ml of peripheral blood in each tube following the manufacturer’s recommended protocol (PaxGene Blood RNA Kit Handbook, Qiagen). The quality and integrity of the total RNA was evaluated on the 2100 Bioanalyzer (Agilent Technologies) and the concentration was measured using a NanoDrop spectrophotometer (NanoDrop Technologies). Quality criteria included a 28S/18S ratio as measured by the Bioanalyzer of at least 1.2, and a total RNA yield of at least 3 µg.
|
| Label |
biotin
|
| Label protocol |
cDNA synthesis, amplification and biotin labeling were performed according to the manufacturer’s instructions using the Codelink iExpress reagent kit (cat. No. 67601000).
|
| |
|
| Hybridization protocol |
Hybridisation was performed according to the manufacturer’s instructions.
|
| Scan protocol |
The slides were scanned with a MicroArray Scanner G2505B scanner (Agilent Technologies) and the image was quantified with CodeLink Expression software (version 4.2).
|
| Description |
none
|
| Data processing |
Raw intensities were background subtracted, set on 0.5 when result was negative and normalized with the Cyclic Loess method using the Codelink package in the Bioconductor R software. After normalization, we used log2-transformed expression intensities for all subsequent analyses.
|
| |
|
| Submission date |
Jun 19, 2009 |
| Last update date |
Sep 04, 2011 |
| Contact name |
Willemijn M Passtoors |
| E-mail(s) |
w.m.passtoors@lumc.nl
|
| Organization name |
Leiden University Medical Center
|
| Department |
Molecular Epidemiology
|
| Street address |
P.O. Box 9600
|
| City |
Leiden |
| State/province |
Zuid-Holland |
| ZIP/Postal code |
2300 RC |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
| GSE16717 |
Gene expression profiles in the Leiden Longevity Study |
|
