|
| Status |
Public on Apr 07, 2020 |
| Title |
L32_2 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood, CD8+ sorted
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: Non-small cell lung cancer
tissue: Blood
cell type: CD8+
treatment: Post-treatment (nivolumab)
individual: L32
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells using RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The cRNA was amplified, labeled using GeneChip WT Pico Reagent Kit (ThermoFisher) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to Clariom D Assay human array (Thermofisher), washed and stained according to the manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner.
|
| Data processing |
The data were analyzed with median polish summarization and quantile normalization by Affymetrix® Transcriptome Analysis Console Software, and obtained normalized intensity values.
|
| |
|
| Submission date |
Dec 04, 2019 |
| Last update date |
Apr 07, 2020 |
| Contact name |
Kenji Chamoto |
| Organization name |
Graduate School of Medicine, Kyoto University
|
| Department |
Department of Immunology and Genomic Medicine
|
| Street address |
Yoshida Konoe-cho, Sakyo-ku
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE141479 |
Combination of host immune metabolic biomarkers for the PD-1 blockade cancer immunotherapy |
|
