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Status |
Public on Apr 07, 2020 |
Title |
L58_1 |
Sample type |
RNA |
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|
Source name |
Peripheral blood, CD8+ sorted
|
Organism |
Homo sapiens |
Characteristics |
diagnosis: Non-small cell lung cancer tissue: Blood cell type: CD8+ treatment: Pre-treatment individual: L58
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from cells using RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
The cRNA was amplified, labeled using GeneChip WT Pico Reagent Kit (ThermoFisher) according to the manufacturer's instructions.
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|
|
Hybridization protocol |
cRNA was hybridized to Clariom D Assay human array (Thermofisher), washed and stained according to the manufacturer's instructions.
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Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner.
|
Data processing |
The data were analyzed with median polish summarization and quantile normalization by Affymetrix® Transcriptome Analysis Console Software, and obtained normalized intensity values.
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|
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Submission date |
Dec 04, 2019 |
Last update date |
Apr 07, 2020 |
Contact name |
Kenji Chamoto |
Organization name |
Graduate School of Medicine, Kyoto University
|
Department |
Department of Immunology and Genomic Medicine
|
Street address |
Yoshida Konoe-cho, Sakyo-ku
|
City |
Kyoto |
State/province |
Kyoto |
ZIP/Postal code |
606-8501 |
Country |
Japan |
|
|
Platform ID |
GPL23126 |
Series (1) |
GSE141479 |
Combination of host immune metabolic biomarkers for the PD-1 blockade cancer immunotherapy |
|