|
| Status |
Public on Nov 21, 2009 |
| Title |
Sarcoma_12 |
| Sample type |
RNA |
| |
|
| Source name |
Soft Tissue Sarcoma
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Soft Tissue Sarcoma
genotype: LSL-KrasG12D; Trp53Fl/Fl
|
| Treatment protocol |
Tumors were isolated from surrounding normal tissue.
|
| Growth protocol |
Tumors were induced via injection of Adeno-Cre ~3 months prior to surgical removal of the tumor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was followed by clean up using the RNeasy kit (Qiagen) per manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Labeled RNA was prepared from 2 µg total RNA using one-cycle target labeling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hours at 45C on Mouse genome 430A 2.0 arrays and underwent automated washing and staining on the fluidics station.
|
| Scan protocol |
Genechips were scanned using the GeneChip Scanner 3000 7g.
|
| Description |
Gene expression data from soft tissue sarcomas compared to normal muscle isolated from mice without tumors.
|
| Data processing |
Data was normalized using the RMA algorithm.
|
| |
|
| Submission date |
Jun 24, 2009 |
| Last update date |
Nov 10, 2009 |
| Contact name |
Jeffrey Mito |
| Organization name |
Duke University Medical Center
|
| Street address |
LSRC B232, Box 91006
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE16779 |
Undifferentiated Pleomorphic Sarcoma Model |
|
