|
| Status |
Public on Feb 08, 2021 |
| Title |
HeLa_DMSO-c |
| Sample type |
SRA |
| |
|
| Source name |
HeLa
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
treatment: DMSO
|
| Treatment protocol |
Incubated with 10 μM ALESIA racemate in MEM containing 5% fetal bovine serum and 1% penicillin-streptomycin for 6 hours, and then harvested for extraction of RNA
|
| Growth protocol |
HeLa cells were expanded in MEM containing 10% fetal bovine serum and 1% penicillin-streptomycin.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted from HeLa cells using the RNeasy mini kit (QIAGEN).
TruSeq Stranded mRNA Library Prep Kit
Oligo-dT beads capture polyA tails
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Basecalling was performed with NovaSeq Control Software v1.4.0
The seuqence data was mapped to the genome sequence with STAR ver. 2.4.1d .
Genome_build: hg19
Supplementary_files_format_and_content: Expression values of refseq gene models were calculated with RSEM ver. 1.3.0.
|
| |
|
| Submission date |
Jan 14, 2020 |
| Last update date |
Feb 08, 2021 |
| Contact name |
Kei IIDA |
| E-mail(s) |
kiida@life.kindai.ac.jp
|
| Organization name |
Kindai University
|
| Street address |
Kowakae 3-4-1
|
| City |
Higashi-Osaka |
| State/province |
Osaka |
| ZIP/Postal code |
5778502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE143668 |
S1PR3–G12-biased agonist ALESIA targets cancer metabolism and promotes glucose starvation [HeLa RNA-seq] |
| GSE143670 |
S1PR3–G12-biased agonist ALESIA targets cancer metabolism and promotes glucose starvation |
|
| Relations |
| BioSample |
SAMN13862610 |
| SRA |
SRX7556728 |
