|
| Status |
Public on Jan 17, 2021 |
| Title |
PDLDS |
| Sample type |
RNA |
| |
|
| Source name |
PDLDS
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: periodontal ligament cells
patient diagnosis: Down syndrome
|
| Growth protocol |
Both PDLGO and PDLDS were cultured in the presence of 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extract protocol not provided
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared from 100 ng of total RNA using GeneChip WT PLUS Reagent Kit（Thermo Fisher Scientific).
|
| |
|
| Hybridization protocol |
Following fragmentation, 2 μg of Single-Stranded cDNA was hybridized for 16 hrs at 45˚C on Clariom S Array, Human. Arrays was washed and stained in the GeneChip Fluidics Station 450 (Thermo Fisher Scientific).
|
| Scan protocol |
Clariom S array was scanned using GeneChip Scanner 3000 7G.
|
| Data processing |
The data were analyzed with Expression Console Software 1.4.1 offered SST-RMA for gene level analysis using Thermo Fisher Scientific default analysis settings.
|
| |
|
| Submission date |
Jan 17, 2020 |
| Last update date |
Jan 17, 2021 |
| Contact name |
Asakawa Takeyoshi |
| E-mail(s) |
tasakawa1116@gmail.com, tasakawa@dent.showa-u.ac.jp
|
| Phone |
+81037871151
|
| Organization name |
Showa university
|
| Department |
Pediatric
|
| Street address |
2-1-1 Kitasenzoku
|
| City |
Tokyo |
| State/province |
Ota-ku |
| ZIP/Postal code |
145-8515 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE143885 |
Gene expression profiling in periodontal ligament cells derived from Down syndrome |
|
