|
| Status |
Public on May 18, 2020 |
| Title |
O_CCA_63 |
| Sample type |
RNA |
| |
|
| Source name |
Urine EVs
|
| Organism |
Homo sapiens |
| Characteristics |
disease: Cholangiocarcinoma
|
| Growth protocol |
NHC, H69, EGI and TFK cell lines were cultured in collagen-coated plates: NHC, H69 and EGI cells in FLASK media (as described in DOI: 10.1136/gutjnl-2013-305281) and TFK cells in DMEM/F12+Glutamax media suplemented with FBS10%
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from the described samples by using miRCURY RNA Isolation kit - Biofluids (EXIQON; ref #300112). Quality control was performed with 2100 Bioanalyzer.
|
| Label |
Biotin
|
| Label protocol |
Whole-Genome Gene Expression DASL®HT Assay Guide (Part#15018210 Rev. D)
|
| |
|
| Hybridization protocol |
Standard, as recommended by illumina
|
| Scan protocol |
Standard, as recommended by illumina
|
| Description |
CCA
O_CCA_63
|
| Data processing |
The data was preprocessed by Bioconductor lumi package (version 2.22.1). It was log2 transformed and quantile normalized. The normalized data table does not contain all the probes since the ones that do not follow the established criteria are filtered out. Only probes with a detection p-value lower than 0.01 in at least one sample are analzyed.
|
| |
|
| Submission date |
Jan 30, 2020 |
| Last update date |
May 18, 2020 |
| Contact name |
Ana Maria Aransay |
| E-mail(s) |
amaransay@cicbiogune.es
|
| Phone |
0034944061325
|
| Organization name |
CIC bioGUNE
|
| Department |
Genome Analysis Platform
|
| Street address |
Parque tecnologico de Bizkaia, Building 801-A
|
| City |
Derio |
| State/province |
BIZKAIA |
| ZIP/Postal code |
48160 |
| Country |
Spain |
| |
|
| Platform ID |
GPL14951 |
| Series (1) |
| GSE144521 |
RNA profiles in serum and urine extracellular vesicles of patients with cholangiocarcinoma mimicking tumor expression: novel liquid biopsy approach |
|
