|
| Status |
Public on Jun 01, 2020 |
| Title |
leukemic cell line IMS_M2 treated with ATRA replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
leukemic cell line IMS_M2 treated with ATRA
|
| Organism |
Homo sapiens |
| Characteristics |
drug: ATRA
cell line: IMS_M2
replicate number: 1
|
| Treatment protocol |
Comparison of drugs ATO+ATRA, ATO, ATRA, JQ1, OTX015 and DMSO in IMS-M2 and OCI AML3 leukemic cell lines
|
| Growth protocol |
24h, 37°, CO2 = 5%, in RPMI 10/20% SVF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were extracted using a promega extractor according to manufacturer protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix manual target preparation for genechip ® whole transcript expression array.
|
| |
|
| Hybridization protocol |
Incubated with rotation at 60 RPM for 16 hours at 45C
|
| Scan protocol |
Scanned with the Affymetrix® GeneChip Scanner 3000 7G using the Command Console software (Affymetrix®)
|
| Description |
IMS_M2__ATRA__1
|
| Data processing |
Analyzed using the Affymetrix® rma-sketch routine.
Log2 rma-sketch values computed with apt Affymetrix software
|
| |
|
| Submission date |
Mar 04, 2020 |
| Last update date |
Jun 02, 2020 |
| Contact name |
Marc Delord |
| E-mail(s) |
mdelord@gmail.com
|
| Organization name |
Centre hospitalier de Versailles
|
| Department |
Recherche clinique
|
| Street address |
Hopital Saint-Louis, 1 avenue Claude Vellefaux
|
| City |
Paris |
| ZIP/Postal code |
75010 |
| Country |
France |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE146362 |
Comprehensive comparison and biologic activity of novel therapeutic agents in NPM1 mutated Acute Myeloid Leukemia (AML) |
|
