|
| Status |
Public on Apr 01, 2021 |
| Title |
Hip_CFA_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampus, CFA, replicate 3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: hippocampus
Sex: male
age: 12 weeks
treatment: CFA
|
| Treatment protocol |
To induce chronic inflammatory pain, 10 μl of 50% CFA was injected subcutaneously into the dorsal surface of the left hindpaw.
|
| Growth protocol |
Mice were housed in plastic cages containing wood chips and covered with a wire lid. The cages were kept at 23±2 °C under a 12 h light/dark cycle (lights on at 8:00). The diet were standard laboratory food and water ad libitum. Male mice of 12 weeks old were used in this study.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Two days after CFA injection, the mice were deeply anesthetized with a medetomidine-midazolam-butorphanol combination and were transcardially perfused with cold PBS. The brains were then quickly removed and cut into coronal slices with brain matrix. The hippocampus and anterior cingulate cortex (ACC) were punched out from the coronoal slices and preserved in RNAlater (Ambion) solution according to the manufacturer's instructions and were stored at -80℃ for further processing.
Total RNA was extracted from each source using RNeasy Lipid Tissue Mini Kit (QIAGEN) according to the manufacturer's protocol. 100 ng of total RNA was subsequently carried out to First-strand cDNA synthesis using GeneChip® WT PLUS Reagent Kit (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Fragmented single-strand cDNA were subjected to 3' terminal labeling with biotin using GeneChip® WT PLUS Reagent Kit in accordance with manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Fragmented and biotin-labeled single-strand cDNA (5.5 μg each) hybridized on GeneChip® MTA 1.0 for 16 h at 45°C with rotation at 60 rpm using GeneChip® Hybridization Oven 645.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G (Affymetrix).
|
| Description |
Hip CFA 3
|
| Data processing |
The data were analyzed with Expression Console version 1.4.1 (Affymetrix) using default settings for background correction, normalization and summarization. CHP files include RMA values.
|
| |
|
| Submission date |
Mar 19, 2020 |
| Last update date |
Apr 02, 2021 |
| Contact name |
Shuji Shimoyama |
| E-mail(s) |
shuji-s@hirosaki-u.ac.jp
|
| Phone |
+81-172-39-5138
|
| Organization name |
Hirosaki University Graduate School of Medicine
|
| Department |
Department of Neurophysiology
|
| Street address |
5 Zaifu-cho
|
| City |
Hirosaki |
| State/province |
Aomori |
| ZIP/Postal code |
036-8562 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21877 |
| Series (1) |
| GSE147216 |
The expression profiling of the anterior cingulate cortex and hippocampus in chronic inflammatory pain mice |
|
