|
| Status |
Public on Mar 24, 2020 |
| Title |
WT_capFb_1 |
| Sample type |
SRA |
| |
|
| Source name |
FACS-sorted primary cell
|
| Organism |
Mus musculus |
| Characteristics |
cell type: thymic capsular fibroblast (capFb)
strain: C57BL/6
genotype: wild type
age: 6-week-old
tissue: thymus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were collected by FACS sorting. Total RNA was extracted using RNeasy micro kit (QIAGEN).
cDNA was synthesized and amplified using SMART-seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Data processing |
Data were acquired on an Ion Proton sequencer (Thermo Fisher) and analyzed using CLC genomics Workbench v12 (CLC) and GeneSpring (Agilent).
Supplementary_files_format_and_content: Excel files include RPKM values for each sample
|
| |
|
| Submission date |
Mar 23, 2020 |
| Last update date |
Mar 25, 2020 |
| Contact name |
Takeshi Nitta |
| Organization name |
Graduate School of Medicine and Faculty of Medicine, The University of Tokyo
|
| Department |
Department of Immunology,
|
| Street address |
7-3-1 Hongo, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18635 |
| Series (1) |
| GSE147357 |
RNA-seq analysis on thymic stromal cells |
|
| Relations |
| BioSample |
SAMN14423691 |
| SRA |
SRX7967710 |
