|
| Status |
Public on Mar 25, 2020 |
| Title |
Muscle_Mid_1014 |
| Sample type |
RNA |
| |
|
| Source name |
Vastus lateralus muscle, midterm
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: muscle
Sex: male
muscle name: Vastus lateralus
time point: midterm
|
| Treatment protocol |
Subjects consumed a 250-mL beverage containing either 29 casein protein or an isocaloric amount of carbohydrate immediately after cessation of each exercise session (3 per wk) and every day before sleep (7 per wk).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the skeletal muscle tissue by using Trizol reagent (Invitrogen, Breda, Netherlands)
|
| Label |
biotin
|
| Label protocol |
Total RNA was labelled using an Affymetrix WT plus reagent (Life Technologies, Bleiswijk, Netherlands)
|
| |
|
| Hybridization protocol |
Total RNA was hybridized to human whole genome Genechip Human Gene 2 (Life Technologies, Bleiswijk, Netherlands)
|
| Scan protocol |
Sample labelling, hybdridization to chips, and image scanning were performed according manufacturer's instructions (Life technologies, Bleiswijk, Netherlands)
|
| Description |
Gene expression data from human muscle tissue
G314_B05_041_1014_M.CEL (M_P; p1014)
|
| Data processing |
The data were analyzed using limma R library
|
| |
|
| Submission date |
Mar 24, 2020 |
| Last update date |
Mar 25, 2020 |
| Contact name |
Pim Knuiman |
| E-mail(s) |
p.knuiman@leeds.ac.uk
|
| Phone |
01133430842
|
| Organization name |
University of Leeds
|
| Department |
Biological Sciences
|
| Street address |
Clarendon Way
|
| City |
Leeds |
| ZIP/Postal code |
LS2 9JT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL28236 |
| Series (1) |
| GSE147494 |
Impact of protein on muscle transcriptome to endurance training |
|
