|
| Status |
Public on Jan 24, 2021 |
| Title |
P30 |
| Sample type |
SRA |
| |
|
| Source name |
lung
|
| Organism |
Homo sapiens |
| Characteristics |
age: 73
gender: male
|
| Treatment protocol |
Prior to tissue dissociation, the specimens were washed with Hanks Balanced Salt Solution (HBSS) for three times and minced into 1–2 mm pieces. The tissue pieces were digested in 2ml GEXSCOPETM Tissue Dissociation Solution (Singleron Biotechnologies) at 37°C for 15min in a 15ml centrifuge tube with continuous agitation
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
The concentration of single-cell suspension was adjusted to 1×105 cells/mL in PBS. Single cell suspension was then loaded onto a microfluidic chip (part of Singleron GEXSCOPETM Single Cell RNAseq Kit, Singleron Biotechnologies) and single cell RNA-seq libraries were constructed according to the manufacturer’s instructions (Singleron Biotechnologies).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Quality control
scRNA-seq reads were aligned to the GRCh38 genome assembly using STAR version 020201
aligned bam files were dealing with Featurecounts Version 1.6.2 to get UMI counts of each cell
Genome_build: GRCh38
Supplementary_files_format_and_content: expression matrix file using RNA raw count
|
| |
|
| Submission date |
Apr 03, 2020 |
| Last update date |
Feb 10, 2021 |
| Contact name |
Yixin Li |
| E-mail(s) |
liyixin@singleronbio.com
|
| Organization name |
singleron
|
| Street address |
No.218,Xinghu Street
|
| City |
Suzhou |
| ZIP/Postal code |
215028 |
| Country |
China |
| |
|
| Platform ID |
GPL20795 |
| Series (1) |
| GSE148071 |
Comprehensive Profiling of Cancer Cells and Their Microenvironment in Advanced NSCLC |
|
| Relations |
| BioSample |
SAMN14534724 |
