|
| Status |
Public on Sep 17, 2010 |
| Title |
KJ201_CaCl2/FK506_30m_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
KJ201_CaCl2/FK506_30m_rep4
|
| Organism |
Pyricularia oryzae |
| Characteristics |
tissue: mycelia
developmental stage: young
genome/variation: wild type
|
| Treatment protocol |
After thorough washing with sterile distilled water, the mycelia were treated with 200mM CaCl2 with or without 10ug/ml FK506 for 30m with shaking.
|
| Growth protocol |
Mycelial blocks from actively growing margin of colony were inoculated into complete media (CM) liquid media at 25C by shaking for 3 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen mycelial powder using an Easy-Spin RNA extraction kit (iNtRON Biotechnology, Seoul, Korea).
|
| Label |
Cy3
|
| Label protocol |
Five hundred nanograms of total RNA was converted into labeled cRNA with nucleotides coupled to fluorescent dye Cy3 using the Quick Amp Kit (Agilent Technologies, Palo Alto, CA) following the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Cy3-labeled cRNA (1.65 ug) from each sample was hybridized to Magnaporthe grisea 2.0 4x44k Microarrays.
|
| Scan protocol |
The hybridized array was then washed and scanned and data was extracted from the scanned image using Feature Extraction version 10.2 (Agilent Technologies).
|
| Description |
Gene expression in wild type mycelia after CaCl2/FK506 treatment
|
| Data processing |
An error-weighted average signal intensity of two probes within a chip was used for normalization with Lowess normalization module implanted in JMP Genomics software. An average expression of all probes among 16 data sets was used as the baseline.
|
| |
|
| Submission date |
Sep 21, 2009 |
| Last update date |
Sep 21, 2009 |
| Contact name |
Soonok Kim |
| E-mail(s) |
kim.2527@osu.edu
|
| Phone |
614-247-0012
|
| Fax |
614-292-4455
|
| Organization name |
The Ohio State University
|
| Department |
Plant Pathology
|
| Street address |
2021 Coffey Rd.
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL9203 |
| Series (2) |
| GSE18185 |
Ca2+/calcineurin/CRZ1 dependent gene expression in Magnaporthe oryzae |
| GSE18193 |
ChIP-chip and gene expression model of the MoCRZ1 mediated circuit for Ca2+/calcineurin signaling in rice blast fungus |
|
