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Status |
Public on Mar 31, 2011 |
Title |
CMP sample 1 |
Sample type |
RNA |
|
|
Source name |
Lin(CD3e; CD4, CD8a, CD19, CD45R, CD127; Gr1, TER119)- Sca1- CD117+ CD34+/low CD16/32-low
|
Organism |
Mus musculus |
Characteristics |
genetic background: C57Bl6 cell type: CMP
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction following manufacturer's protocol.
|
Label |
Biotin
|
Label protocol |
10 to 50 nanograms of total RNA was amplified and processed to produce biotinylated cDNA targets using two round of in vitro RNA synthesis
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|
|
Hybridization protocol |
3.75 micrograms of biotinylated material was hybridized according to the standard Affymetrix protocol.
|
Scan protocol |
Standard Affymetrix protocol
|
Description |
purified Lineage- Sca1- CD117+ CD34+/low CD16/32-low cells form 8 week old female mice
|
Data processing |
RMA background correction and quantile normalization done with the GenePattern ExpressionFileCreator module (http://www.broad.mit.edu/cancer/software/genepattern/) which uses the Bioconductor implementation of RMA (http://www.bioconductor.org/). This function computes the Robust Multichip Average expression measure described in Irizarry et al Biostatistics (2003).
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|
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Submission date |
Oct 08, 2009 |
Last update date |
Mar 31, 2011 |
Contact name |
Andrei V. Krivtsov |
E-mail(s) |
andrei.krivtsov@childrens.harvard.edu
|
Phone |
617 9192501
|
Fax |
617 730 0934
|
Organization name |
Children's Hospital
|
Department |
Hem Onc
|
Street address |
1 Blackfan circle
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL8321 |
Series (1) |
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