|
| Status |
Public on Oct 21, 2009 |
| Title |
P14 WT FoxJ1EGFP+ |
| Sample type |
RNA |
| |
|
| Source name |
P14 FoxJ1-EGFP+ tissue FoxJ1 wildtype
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brain/ependymal layer
genotype: Wildtype
age: postnatal day 14
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mouse brains were rapidly collected in 10% RNAlater on ice, frozen, and laser capture microscopy was used to isolate FoxJ1-EGFP domains from wildtype and FoxJ1 null brains.
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug cDNA was hybridized for 16 hr at 45C on the Mouse Gene 1.0 ST. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
Gene expression data from laser captured FoxJ1-EGFP tissue in postnatal day 14 mouse brain
|
| Data processing |
RMA
|
| |
|
| Submission date |
Oct 21, 2009 |
| Last update date |
Oct 21, 2009 |
| Contact name |
Troy Ghashghaei |
| E-mail(s) |
troy_ghashghaei@ncsu.edu
|
| Phone |
919-513-6174
|
| Fax |
919-513-6465
|
| URL |
http://cvm.ncsu.edu/mbs/personnel/ghashghaei_troy.html
|
| Organization name |
North Carolina State University
|
| Department |
Molecular Biomedical Sciences
|
| Lab |
Ghashghaei
|
| Street address |
4700 Hillsborough Street
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27606 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE18678 |
Expression data from ependymal layer of wild type and FoxJ1 null postnatal male mice |
|
