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Sample GSM465086 Query DataSets for GSM465086
Status Public on Oct 30, 2009
Title ODN2395 2
Sample type RNA
 
Source name 2h treatment of MLE-15 cells
Organism Mus musculus
Characteristics cell type: MLE-15
Treatment protocol MLE-15 cells were treated with PBS, ODN2395 (0.4 ug), Pam2CSK4 (0.2 ug) or ODN2395+Pam2CSK4 for 2 hours. For each, a 20 ul volume of the treatment was added to a 500 ul volume of RPMI for each well of 6-well tissue culture plates.
Growth protocol MLE-15s grown in monolayer to approximately 80% confluence in 6 well plates
Extracted molecule total RNA
Extraction protocol total RNA extracted using Qiagen Rneasy per manufacturers instructions. cRNA amplification using Ambion total prep kit per manufacturers instructions.
Label Cy3
Label protocol cRNA was hybridized to BeadChips prior to labeling with Cy3-Streptavidin per Illumina instructions.
 
Hybridization protocol cRNA was hybridized to BeadChips per Illumina instructions.
Scan protocol Chips were scanned using level 3 signal using a BeadStation 500 scanner.
Description ODN2395 treated
Data processing Data was processed using Illumina BeadStudio v3. Matrix tables were generated both without normalization and with rank invariant normalization, using sham group as the reference group.
 
Submission date Oct 23, 2009
Last update date Oct 29, 2009
Contact name Scott E Evans
E-mail(s) seevans@mdanderson.org
Organization name University of Texas-M.D. Anderson Cancer Center
Department Pulmonary Medicine - Box 403
Street address 1400 Holcombe Blvd
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6885
Series (1)
GSE18725 Gene expression in respiratory epithelial cells treated to induce resistance with synthetic TLR ligands

Data table header descriptions
ID_REF
VALUE Values reported were derived using Illumina BeadStudio v3 and are relative signal intensity normalized to background.

Data table
ID_REF VALUE
ILMN_2896528 205.0361
ILMN_2721178 34.2981
ILMN_3033922 2059.456
ILMN_3092673 7686.839
ILMN_2816356 129.3659
ILMN_2808939 1023.73
ILMN_2634564 566.0436
ILMN_2737647 7.720787
ILMN_2734484 488.8838
ILMN_2952292 112.2372
ILMN_2699078 -4.184708
ILMN_1213681 533.8113
ILMN_2735413 11.7566
ILMN_2735415 18.78982
ILMN_2891688 729.5838
ILMN_2637698 2221.854
ILMN_2674228 784.6387
ILMN_2601546 10.33876
ILMN_1230831 9.35545
ILMN_2848071 29.23899

Total number of rows: 25697

Table truncated, full table size 557 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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