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Sample GSM465451 Query DataSets for GSM465451
Status Public on Jan 19, 2010
Title A435_3_LPS_Luteolin
Sample type RNA
 
Source name A435_3_LPS_Luteolin
Organism Mus musculus
Characteristics agent: LPS and luteolin
Treatment protocol BV-2 cells were left untreated or stimulated for 24h with 50 µM Luteolin, 50 ng/ml LPS, or 50 µM Luteolin + 50 ng/ml LPS
Growth protocol BV-2 cells were cultured as described in Blasi et al. J Neuroimmunol. 1990 May;27(2-3):229-37.
Extracted molecule total RNA
Extraction protocol Total RNA from the PUER cells was extracted according to the manufacter's instructions using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA)
Label Biotin
Label protocol Biotinylated sense-strand DNA for hybridization to Affymetrix expression arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using 300ng total RNA and standard method according to the manufacturer’s protocols (Affymetrix, GeneChip WT terminal labelling kit 900671).
 
Hybridization protocol The labeling assay yielded labeled single-stranded DNA that was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays (expression arrays) according to manufacturer’s protocols at the KFB Regensburg
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 Blank7G (Affymetrix).
Description Gene expression data from BV-2 cells treated with 50 ng/ml LPS + 50 µM Luteolin
Data processing RMA
 
Submission date Oct 26, 2009
Last update date Mar 29, 2010
Contact name Thomas Langmann
E-mail(s) thomas.langmann@klinik.uni-regensburg.de
Phone +49-941-944-5423
Fax +49-941-944-5402
Organization name Institute of Human Genetics
Department University of Regensburg
Street address Franz-Josef-Strauss-Allee 11
City Regensburg
ZIP/Postal code 93042
Country Germany
 
Platform ID GPL1261
Series (1)
GSE18740 Luteolin has anti-inflammatory and neurotrophic effects on microglia

Data table header descriptions
ID_REF
VALUE RMA linear signal intensity

Data table
ID_REF VALUE
1415670_at 885.272
1415671_at 3891.54
1415672_at 2672.82
1415673_at 1511.832
1415674_a_at 1266.527
1415675_at 1414.585
1415676_a_at 4714.133
1415677_at 770.3192
1415678_at 1773.039
1415679_at 3235.985
1415680_at 1407.524
1415681_at 3374.52
1415682_at 442.797
1415683_at 3243.706
1415684_at 541.342
1415685_at 1663.296
1415686_at 744.746
1415687_a_at 11177.48
1415688_at 2039.322
1415689_s_at 394.9127

Total number of rows: 45101

Table truncated, full table size 894 Kbytes.




Supplementary file Size Download File type/resource
GSM465451_A435_3_LPS_Luteolin.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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