|
| Status |
Public on Jun 22, 2021 |
| Title |
QSC Cont Biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Freshly isolated muscle satellite cells from Pax7-CreERT2::Calcr+/+::Rosa-YFP mice
|
| Organism |
Mus musculus |
| Characteristics |
age: 10 weeks
genotype: Pax7Cre/+::Calcr +/+::Rosa-YFP/YFP
cell type: murine muscle satellite cells
|
| Treatment protocol |
Tamoxifen was treated
|
| Growth protocol |
These samples were directly isolated from mouse skeletal muscle. Therefore, the cells were not cultured in vitro.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Used Qiagen Rneasy Mini Kit according to instructions therein.
|
| Label |
biotin
|
| Label protocol |
GeneChip® Eukayotic Small Sample Target Labeling Assay Version II.
|
| |
|
| Hybridization protocol |
Following fragmentation, labeled cRNA were hybridized for 16 hr at 45Cº on GeneChip Mouse Genome Array (430 2.0). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from control muscle satellite cells
|
| Data processing |
Affymetrix MAS 5.0 with p-value cuttoffs of 0.04 and 0.06 for present, marginal, and absent.
|
| |
|
| Submission date |
Jul 09, 2020 |
| Last update date |
Jun 22, 2021 |
| Contact name |
so-ichiro fukada |
| E-mail(s) |
fukada@phs.osaka-u.ac.jp
|
| Organization name |
osaka University
|
| Street address |
1-6 yamadaoka
|
| City |
suita |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE154082 |
Expression data from freshly isolated murine muscle satellite cells |
|
