|
| Status |
Public on Jul 31, 2020 |
| Title |
iHEC_35 |
| Sample type |
SRA |
| |
|
| Source name |
induced hematopoietic endothelia cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: induced hematopoietic endothelia cells
strain: C57BL/6
|
| Treatment protocol |
The single iHECs and iHPCs were sorted for RNAseq analysis.
|
| Growth protocol |
Expression of defined factors Hoxa9 and Runx1 in ESC/iPSC led to the production of an hematopoietic cell type
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
The procedure of RNA extraction and reverse transcription into double-stranded DNA were prepared using Discover-sc WTA Kit V2(Vazyme, N711)
Libraries were prepared according to instruction of TruePrepTM DNA Library Prep Kit V2 (Vazyme,TD503).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Bcl2fastq (verison 2.16.0.10) convert .bcl files into fastq files
Fastq files were aligned to mm10 genome.*.ht2 files to generate *.sam file by HISAT2 (version 2.1.0). And the used samtools (version 0.1.19) to convert and sort the sam file to bam file. StringTie (version 1.3.4) were used to calculated genes expression level in TPM.
Gene expression levels in TPM of different samples were normalized by Stringtie.
Genome_build: mm10
Supplementary_files_format_and_content: TPM table was a csv file
|
| |
|
| Submission date |
Jul 16, 2020 |
| Last update date |
Jul 31, 2020 |
| Contact name |
Jinyong Wang |
| E-mail(s) |
wang_jinyong@gibh.ac.cn
|
| Organization name |
Guangzhou Institutes of Biomedicine and Health (GIBH)
|
| Street address |
190 Kai Yuan Avenue, Science Park, Guangzhou, China
|
| City |
Guangzhou |
| State/province |
GuangDong |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (2) |
| GSE121375 |
T cell regeneration from pluripotent stem cells by defined transcription factors |
| GSE128738 |
T cell regeneration from pluripotent stem cells by defined transcription factors Runx1 and Hoxa9 [single hematopoietic cells (iR9-iHEC&iR9-iHPC) II] |
|
| Relations |
| BioSample |
SAMN15563074 |
| SRA |
SRX8752432 |
