|
| Status |
Public on Nov 05, 2009 |
| Title |
wildtype stomach neonatal replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
stomach
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: mixed SV129/C57BL/6
gender: unknown
age: neonatal
tissue: stomach
genotype: wildtype
|
| Treatment protocol |
Not applicable.
|
| Growth protocol |
Mice were maintained on standard laboratory chow and allowed food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol, per manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
cRNA labeling was carried out using standard Affymetrix protocols.
|
| |
|
| Hybridization protocol |
After fragmentation, labeled cRNA was hybridized to Affymetrix MOE430A expression arrays using standard Affymetrix protocols.
|
| Scan protocol |
Hybridized arrays were scanned using standard Affymetrix protocols.
|
| Description |
neonatal stomach tissue from wild type mice
|
| Data processing |
Data was normalized within MAS5.0. by using the global scaling option using all probe sets. Target intensity of each chip was set to 150 and the normalization factor for each chip was obtained as the factor required to convert the trimmed mean signal (2% trim from the top and bottom) for each chip to 150. Then the raw signal for each gene on that chip was multiplied by the normalization factor to obtain normalized gene signals.
|
| |
|
| Submission date |
Nov 04, 2009 |
| Last update date |
Nov 05, 2009 |
| Contact name |
Andrew Billin |
| E-mail(s) |
andrew.n.billin@gsk.com
|
| Organization name |
GlaxoSmithKline
|
| Street address |
5 Moore Drive
|
| City |
Research Triangle Park |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE18896 |
ERRĪ³ Regulates Cardiac, Gastric and Renal Potassium Homeostasis |
|
