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Sample GSM469235 Query DataSets for GSM469235
Status Public on Jan 01, 2011
Title 600_24_CSA_3
Sample type RNA
 
Source name IMCD cultivated at 300 mosmol/kg challenged for 24h with 600 mosmol/kg CSA rep.3
Organism Rattus norvegicus
Characteristics tissue: Renal inner medulla
cell type: collecting duct cells
culture condition: 300 mosmol/kg
treatment group: challenged for 24h with 600 mosmol/kg + Cyclosporin A
Treatment protocol Treated with 5 µM Cyclosporin A for 24h as indicated.
Growth protocol Rat primary cultured inner medullary collecting duct cells cultivated at different osmolalities
Extracted molecule total RNA
Extraction protocol Extraction of total RNA was performed using Qiagen RNeasy kit according to the manufacturer's instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with GeneChip Rat Gene 1.0 ST Arrays (Affymetrix) and scanned at the University Hospital Muenster IFG Microarray Core Facility.
Scan protocol Affymetrix Gene ChIP Scanner 3000 7G was used.
Description Rat primary cultured inner medullary collecting duct cells
Data processing Data were processed using XRAY software. Background correction were applied to probe intensities, gene level expression were summarized from the corrected intensities and then quantile normalized and log transformed.
 
Submission date Nov 09, 2009
Last update date Jan 01, 2011
Contact name Bayram Edemir
E-mail(s) bayram.edemir@uk-halle.de
Phone +493455574980
Organization name University Hospital Halle (Saale)
Street address Ernst-Grube-Str. 20
City Halle
ZIP/Postal code 06120
Country Germany
 
Platform ID GPL6247
Series (1)
GSE18945 Tonicity iduced changes in gene expression in IMCD cells and the effect of Cyclosporin A

Data table header descriptions
ID_REF
VALUE Quantile normalized (XRAY) signal intensity

Data table
ID_REF VALUE
10800328 3.302
10874458 2.889
10834373 2.742
10763430 6.251
10883125 5.351
10806375 4.916
10794685 4.453
10781216 2.269
10773004 2.248
10919076 4.254
10850056 3.035
10791677 5.581
10929562 4.202
10875245 6.318
10852174 4.401
10809457 2.641
10933042 6.021
10783378 2.188
10798513 3.638
10732644 2.024

Total number of rows: 28328

Table truncated, full table size 411 Kbytes.




Supplementary file Size Download File type/resource
GSM469235.CEL.gz 6.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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