|
| Status |
Public on Aug 21, 2020 |
| Title |
Hippocampus-Dep-Sus-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampus-Dep-Sus-rep3
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Hippocampus
gender: male
strain: Sprague-Dawley
group: depression-susceptible
|
| Treatment protocol |
According to baseline sucrose intake, animals were divided randomly into stress and control groups. We exposed the stress group to eight weeks of CMS, while the control group was free of CMS.
|
| Growth protocol |
Male Sprague-Dawley albino rats (250 g) were purchased from from the Animal Center of Chongqing Medical University. All the rats were kept under controlled environmental conditions at 21 ± 2 °C and with a 12-h light/dark cycle and fed drinking water and pellet food ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
NanoDrop ND-1000 was used to assess RNA volume and quality.RNA integrity was assessed by standard denatured gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology) with minor modifications. Briefly, mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then, each sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3' bias utilizing a random priming method (Arraystar Flash RNA Labeling Kit, Arraystar). The labeled cRNAs were purified by RNeasy Mini Kit (Qiagen).
|
| |
|
| Hybridization protocol |
The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000. 1 μg of each labeled cRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 °C for 30 min, finally 25 μl 2 × GE Hybridization buffer was added to dilute the labeled cRNA. 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the LncRNA expression microarray slide. The slides were incubated for 17 hours at 65 °C in an Agilent Hybridization Oven. The hybridized arrays were washed, fixed and scanned with using the Agilent DNA Microarray Scanner (part number G2505C).
|
| Scan protocol |
The hybridized arrays were washed, fixed and scanned with using the Agilent DNA Microarray Scanner. (part number G2505C).
|
| Description |
hippocampus tissues of CMS model
|
| Data processing |
Gene expression ratios were determined. If a gene had a P value and expression fold-change value of less than 0.05 and 2.0 respectively, it was deemed differentially expressed.
|
| |
|
| Submission date |
Aug 20, 2020 |
| Last update date |
Aug 21, 2020 |
| Contact name |
Jian Zhou |
| E-mail(s) |
zhoujian@cqmu.edu.cn
|
| Phone |
+862368485763
|
| Organization name |
Chongqing Medical University
|
| Department |
Institute of Neuroscience
|
| Street address |
1 Yixueyuan Road, Yuzhong District
|
| City |
Chongqing |
| State/province |
China |
| ZIP/Postal code |
400016 |
| Country |
China |
| |
|
| Platform ID |
GPL15690 |
| Series (1) |
| GSE156580 |
Intersectional analysis of chronic mild stress-induced lncRNA-mRNA interaction networks in rat hippocampus reveals potential anti-depression/anxiety drug targets |
|
