|
| Status |
Public on Aug 09, 2023 |
| Title |
Muscle_Post_Training_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Muscle biopsy post training derived from subject 54
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: muscle
|
| Treatment protocol |
As described in the paper. Briefly, healthy, sedentary (<120 min of physical activity per week) individuals at high risk for T2D with at least one of the following risk factors (BMI>27 kg/m2, family history (first degree) of T2D, former gestational diabetes) were recruited. Criteria for recruitment and design of the exercise intervention were identical in both studies. 44 individuals (19 of study 1 and 25 of study 2) were included for data analysis. One subject participated in both studies and only data obtained in study 2 were used for further analyses. Severe diseases were excluded by anamnesis including medication, monitoring routine laboratory parameters, electrocardiogram, and physical examination. The participants performed an 8-week supervised aerobic endurance exercise intervention program as described below. Individual training intensity was set at 80% of VO2peak determined before the intervention and kept constant throughout the training program. Insulin sensitivity was assessed after an overnight-fast by 75g-OGTT pre-intervention and 5 days after the last exercise bout; blood samples for clinical routine parameters were obtained before the glucose load. Vastus lateralis muscle biopsies were taken after end of the OGTT before and five days after the 8-week intervention.
|
| Growth protocol |
no
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using the miRneasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
WT PLUS Reagent Kit (Thermo Fisher Scientific).
|
| |
|
| Hybridization protocol |
According to the Thermo Fisher Scientific expression protocol (HWS kit)
|
| Scan protocol |
According to the Thermo Fisher Scientific expression protocol (GeneChip Scanner 3000 7G)
|
| Description |
Gene expression data from muscle biopsy post training derived from subject 54
|
| Data processing |
Transcriptome Analysis Console (TAC; version 4.0.0.25; Thermo Fisher Scientific) was used for quality control and to obtain annotated normalized SST-RMA gene-level data
|
| |
|
| Submission date |
Nov 18, 2020 |
| Last update date |
Aug 09, 2023 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23159 |
| Series (2) |
| GSE161749 |
Acute and long-term exercise adaptation of adipose tissue and skeletal muscle in humans: a matched transcriptomics approach after 8-week training-intervention |
| GSE224310 |
Acute versus long-term exercise adaptation of adipose tissue and skeletal muscle in humans: A matched transcriptomics approach after 8-week training-intervention |
|
