|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Nov 21, 2020 |
Title |
Mouse Rbp4+ PFC/OFC cells LLJL12_L12_S285 |
Sample type |
SRA |
|
|
Source name |
medial prefrontal cortex
|
Organism |
Mus musculus |
Characteristics |
genotype: Rbp4-cre;Ai14
|
Extracted molecule |
total RNA |
Extraction protocol |
mPFC or OFC was dissected form acute slices of the mouse brain. Samples were digested and cells released by tirturation. TdTomato+ cells were FACS sorted into 384 well plates smartseq2
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
LLJL12_L12_S285
|
Data processing |
Basecalling through Illumina pipeline STAR alignment to (I) mouse genome mm10 Count reads in exons using Htseq Produce count matrix Analyse in Seurat Genome_build: mm10 Supplementary_files_format_and_content: seurat v3 object containing count matrix, dimensionality reduction, cluster assignment, metadata
|
|
|
Submission date |
Nov 20, 2020 |
Last update date |
Nov 21, 2020 |
Contact name |
Justus M Kebschull |
Organization name |
Stanford University
|
Street address |
327 Campus Drive
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE161936 |
Single cell RNAseq of Rbp4cre+ neurons from prefrontal cortex |
|
Relations |
BioSample |
SAMN16860391 |
SRA |
SRX9542383 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
|
|
|
|
|