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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 21, 2020 |
| Title |
Mouse Rbp4+ PFC/OFC cells LLJL12_L13_S286 |
| Sample type |
SRA |
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| Source name |
medial prefrontal cortex
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| Organism |
Mus musculus |
| Characteristics |
genotype: Rbp4-cre;Ai14
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| Extracted molecule |
total RNA |
| Extraction protocol |
mPFC or OFC was dissected form acute slices of the mouse brain. Samples were digested and cells released by tirturation. TdTomato+ cells were FACS sorted into 384 well plates
smartseq2
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
LLJL12_L13_S286
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| Data processing |
Basecalling through Illumina pipeline
STAR alignment to (I) mouse genome mm10
Count reads in exons using Htseq
Produce count matrix
Analyse in Seurat
Genome_build: mm10
Supplementary_files_format_and_content: seurat v3 object containing count matrix, dimensionality reduction, cluster assignment, metadata
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| Submission date |
Nov 20, 2020 |
| Last update date |
Nov 21, 2020 |
| Contact name |
Justus M Kebschull |
| Organization name |
Stanford University
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| Street address |
327 Campus Drive
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE161936 |
Single cell RNAseq of Rbp4cre+ neurons from prefrontal cortex |
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| Relations |
| BioSample |
SAMN16860390 |
| SRA |
SRX9542384 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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