|
| Status |
Public on Jun 30, 2021 |
| Title |
54HYO55 |
| Sample type |
RNA |
| |
|
| Source name |
C01P021
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: ALS
tissue: peripheral blood
treatment: 2MIU IL2
time point: Day1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
White blood cell isolation: White blood cells were isolated using LeukoLOCK TM filters
Total RNA extraction: Total RNA was isolated using the LeukoLOCK TM Total RNA isolation kit (AmbionTM) as per manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
200ng of total RNA was used to produce antisense RNA (cRNA) and cDNA which was then fragmented and biotinylated using Affymetrix GeneChip® WT PLUS Kit according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Fragmented and labelled ss cDNA was hybridized on Human Clariom D Affymetrix® chips.
|
| Scan protocol |
The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the AGCC software package to prepare the .CEL files
|
| Description |
Gene expression data from human peripheral blood
|
| Data processing |
Gene expression data was normalised using the signal space transformation robust multi-chip analysis (SST-RMA) method. Data was analysed using TAC and Limma software.
|
| |
|
| Submission date |
Dec 20, 2020 |
| Last update date |
Jun 30, 2021 |
| Contact name |
Ilaria Giovannelli |
| E-mail(s) |
igiovannelli1@sheffield.ac.uk
|
| Organization name |
University of Sheffield
|
| Department |
Sheffield Institute for Translational Neuroscience
|
| Street address |
385a Glossop Road
|
| City |
Sheffield |
| ZIP/Postal code |
S10 2HQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE163560 |
Amyotrophic Lateral Sclerosis Transcriptomics Reveals Immunological Effects of Low-Dose Interleukin-2. |
|
