|
| Status |
Public on Jan 14, 2021 |
| Title |
W_F_004 |
| Sample type |
SRA |
| |
|
| Source name |
lung
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD1
gender: F
genotype: Dll4+/+
tissue: whole Lung
|
| Treatment protocol |
heterozygous compared with wildtype
|
| Growth protocol |
Post natal day 6 (P6)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolation from lung
NEB Library
Whole transcriptome paired end sequencing ( 2 X 150 cycles)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
W_F_004_Dll4+/+
|
| Data processing |
The raw base calling (.bcl) files were converted to demultiplexed compressed FASTQ files using CASAVA
Novogene's STAR was used to map RNA-seq reads against the mouse reference genome
Transcript assembly and abundance estimation and comparing expression were conducted and reported in Fragments Per Kilobase of exon per Million fragments mapped (FPKM).
Genome_build: GRCm38.p4
Supplementary_files_format_and_content: tab-delimited text file of a matrix table generated and reported in Fragments Per Kilobase of exon per Million fragments mapped (FPKM).
|
| |
|
| Submission date |
Jan 13, 2021 |
| Last update date |
Jan 14, 2021 |
| Contact name |
Venkatesh Sampath |
| E-mail(s) |
vsampath@cmh.edu
|
| Organization name |
Children's Mercy Hospital
|
| Department |
Neonatology
|
| Street address |
2401 Gillham Road
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64108 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE164732 |
RNAseq of wild type (WT) and Dll4+/- mouse lung |
|
| Relations |
| BioSample |
SAMN17305266 |
| SRA |
SRX9841738 |
