strain: C57BL/6N tissue: liver age: 17 weeks gender: male genotype: wild type
Treatment protocol
After 2 weeks of treadmill acclimatization of all animals, mice were randomized to 4 groups of n=8 for 6 weeks of dietary intervention and treadmill training: control diet, sedentary (CON_SED); control diet, training (CON_TRAIN); high-energy diet, sedentary (HED_SED); and high-energy diet, training (HED_TRAIN). The high-energy diet (Ssniff (Soest, Germany) E15744-344; 45 kJ% fat, 35 kJ% carbohydrates, 20 kJ% protein; corresponding to Research Diets D12451) contained additional sugar (10 weight% sucrose) and fat (20 weight% lard) and was otherwise similar to the matched control diet (E157453-04 / D12450J; 10 kJ% fat, 70 kJ% carbohydrates, 20 kJ% protein). Chow and tap water were provided ad libitum. Treadmill training was performed 3 times per week, separated by at least one day of rest, for 1 h and during the animals’ active phase, as previously described (Hoene et al., 2009). Treadmill conditions were: After 5 min warmup, 10 m/min at 12° uphill slope in week 1; 11 m/min, 12° in week 2; 12 m/min, 12° in week 3; 12 m/min, 10° in week 4, 12 m/min, 9° in week 5 and 12 m/min, 8° slope in week 6. After 5 weeks, an i.p. glucose tolerance test was performed as previously described, after 16h of fasting and with 1.5 g glucose (20% solution; B. Braun, Melsungen, Germany) per kg body weight. After 6 weeks, mice were analgosedated (150 mg ketamine and 10 mg xylazine /kg body weight) and exsanguinated by decapitation before removal of organs, which were immediately processed or flash-frozen in liquid nitrogen. Both glucose tolerance test and organ collection took place 48h after the preceding training session.
Growth protocol
Male C57BL/6N mice were purchased at an age of 9 weeks from Charles River (Sulzfeld, Germany). Mice were maintained on a 12-h light-dark cycle with free access to water and food.
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated employing the RNeasy Mini kit (Qiagen, Hilden, Germany)
Label
biotin
Label protocol
Total RNA was amplified using the WT PLUS Reagent Kit (Thermo Fisher Scientific Inc., Waltham, USA).
Hybridization protocol
According to the Thermo Fisher Scientific expression protocol (HWS kit)
Scan protocol
According to the Thermo Fisher Scientific expression protocol, Scanner 3000 7G
Description
Gene expression data from liver of mice treated with control diet for 6 weeks, training, biological replicate 4
Data processing
SST-RMA gene-level probeset summary with standard settings of the Thermo Fisher Scientific Expression Console software (v.1.4.1.46)
