|
| Status |
Public on Feb 27, 2021 |
| Title |
miR-100_4 |
| Sample type |
RNA |
| |
|
| Source name |
global microRNA-100 overexpressing mice
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6J
genotype: global miR-100 overexpression using CMV-Cre deleter ( Tg(CMV-cre)1Cgn/J) crossed to miR-100flox/flox, both on C57BL/6J background, Cre positive miR100flox/+ were selected and backcrossed to eliminate the Cre transgene and then further crossed to homozygosity
age: 6 months
Sex: male
tissue: liver
treatment: untreated
|
| Treatment protocol |
Samples from male, 6 months old, untreated mice were used.
|
| Growth protocol |
All mice were kept under a 12h/12h light/dark cycle in a specific pathogen-free animal facility with standard diet and water available ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriPure extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
We used the Affymetrix WT Plus kit for cDNA synthesis, amplification, and second-cycle cDNA synthesis, as well as fragmentation and labeling with Biotin, as described by the manufacturer.
|
| |
|
| Hybridization protocol |
Labeled fragments were hybridized to Affymetrix Mouse Gene 2.0 ST arrays for 16h at 45°C with 60 rpm in an Affymetrix Hybridization oven 645. Arrays were washed and stained using the Affymetrix wash and stain kit as described by the manufacturer.
|
| Scan protocol |
The arrays were scanned with the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from male, 6 months old, untreated mice
|
| Data processing |
CEL-files were produced from the raw data with Affymetrix GeneChip Command Console Software Version 4.0 and standard settings.
CEL-files were processed with Partek Genomics Suite software using standard settings with Anova.
|
| |
|
| Submission date |
Feb 26, 2021 |
| Last update date |
Feb 27, 2021 |
| Contact name |
Christian Smolka |
| E-mail(s) |
christian.smolka@uniklinik-freiburg.de
|
| Phone |
+4976127070460
|
| Organization name |
Heart Center Freiburg University
|
| Department |
Department of Cardiology and Angiology I
|
| Lab |
AG Grundmann
|
| Street address |
Breisacher Strasse 33
|
| City |
Freiburg |
| State/province |
Baden Württemberg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE167603 |
Expression data from global miR-100 overexpressing mice |
|
