|
Status |
Public on Sep 03, 2010 |
Title |
KO-021 |
Sample type |
RNA |
|
|
Source name |
150 pos, CD 48 neg, Lin neg Sca and c-kit pos BM cells
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: Gfi1b KO
|
Treatment protocol |
Gfi1bfl/fl and MxCretg gfi1bfl/fl mice were injected 5 times with 500 µg pIpC every other day for a total of 5 injections.
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen micro-RNA kit was used
|
Label |
biotin
|
Label protocol |
Target RNA was reverse transcribed into cDNA using a NuGEN Ovation Pico RNA Kit and in-vitro transcription was performed to generate biotin-labeled cRNA. cRNA was labeled with streptavidin-phycoerythrin.
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|
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Hybridization protocol |
Samples were hybridized with [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Arrays and scanned using a GeneArray Scanner at the Génome Québec INNOVATION centre of the McGill University, Montréal, QC, Canada
|
Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
Description |
Gfi1b KO
|
Data processing |
Data was processed using the Affymetrix Expression Console software. algorithm-name: rma-gene-default
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Submission date |
Mar 05, 2010 |
Last update date |
Sep 03, 2010 |
Contact name |
Cyrus Khandanpour |
E-mail(s) |
cyrus.khandanpour@uk-essen.de
|
Phone |
+49 20172385212
|
Organization name |
University Hospital Essen
|
Department |
Hematology
|
Street address |
Hufelandstr. 55
|
City |
Essen |
ZIP/Postal code |
45147 |
Country |
Germany |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE20655 |
Effect of loss of Gfi1b on hematopietic stem cells |
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