|
| Status |
Public on Sep 03, 2010 |
| Title |
KO-517 |
| Sample type |
RNA |
| |
|
| Source name |
150 pos, CD 48 neg, Lin neg Sca and c-kit pos BM cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Gfi1b KO
|
| Treatment protocol |
Gfi1bfl/fl and MxCretg gfi1bfl/fl mice were injected 5 times with 500 µg pIpC every other day for a total of 5 injections.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen micro-RNA kit was used
|
| Label |
biotin
|
| Label protocol |
Target RNA was reverse transcribed into cDNA using a NuGEN Ovation Pico RNA Kit and in-vitro transcription was performed to generate biotin-labeled cRNA. cRNA was labeled with streptavidin-phycoerythrin.
|
| |
|
| Hybridization protocol |
Samples were hybridized with [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Arrays and scanned using a GeneArray Scanner at the Génome Québec INNOVATION centre of the McGill University, Montréal, QC, Canada
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
Gfi1b KO
|
| Data processing |
Data was processed using the Affymetrix Expression Console software. algorithm-name: rma-gene-default
|
| |
|
| Submission date |
Mar 05, 2010 |
| Last update date |
Sep 03, 2010 |
| Contact name |
Cyrus Khandanpour |
| E-mail(s) |
cyrus.khandanpour@uk-essen.de
|
| Phone |
+49 20172385212
|
| Organization name |
University Hospital Essen
|
| Department |
Hematology
|
| Street address |
Hufelandstr. 55
|
| City |
Essen |
| ZIP/Postal code |
45147 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE20655 |
Effect of loss of Gfi1b on hematopietic stem cells |
|
