|
| Status |
Public on Mar 01, 2011 |
| Title |
whole-blood_2_Rep47 |
| Sample type |
RNA |
| |
|
| Source name |
whole-blood_2
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Case (2)
|
| Treatment protocol |
Collected blood was aliquoted into PaxGene tubes, inverted 10X and then frozen at -20 C until further processing
|
| Growth protocol |
Whole blood (2.5 ml) was collected via syringe during catheterization.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted per manufacturers protocol (PreAnalytix, Franklin Lakes, NJ)
|
| Label |
Cy3
|
| Label protocol |
200 ng of each patient whole blood RNA was labeled with Cy3 using Agilent's Low RNA Input Linear Amplification Kit following the recommended protocol.
|
| |
|
| Hybridization protocol |
Labeled RNAs were hybridized using the Agilent protocol (Agilent, version 4.0.1)
|
| Scan protocol |
Agilent Feature Extraction Software (version 9.5.3.1) was used for background subtraction and LOWESS normalization.
|
| Description |
n/a
|
| Data processing |
Agilent processed signal values were used; values were scaled to a trimmed mean of 100 using an R script developed in-house at CardioDx.
|
| |
|
| Submission date |
Mar 08, 2010 |
| Last update date |
Mar 01, 2011 |
| Contact name |
James A. Wingrove |
| E-mail(s) |
jwingrove@cardiodx.com
|
| Phone |
650 475-2706
|
| Organization name |
CardioDx, Inc.
|
| Street address |
2500 Faber Place
|
| City |
CA |
| State/province |
CA |
| ZIP/Postal code |
94303 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (2) |
| GSE20680 |
Whole Blood Cell Gene Expression Profiling in Patients with Coronary Artery Disease from the Cathgen Registry |
| GSE20686 |
Whole Blood Cell Gene Expression Profiling in Patients with Coronary Artery Disease (Homo sapiens) |
|
