|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Dec 08, 2021 |
Title |
Gut_Clean_ADKO_rep1 |
Sample type |
SRA |
|
|
Source name |
Gut
|
Organism |
Ixodes scapularis |
Characteristics |
tissue: gut treatment: clean treatment: AdipoR dsRNA
|
Extracted molecule |
total RNA |
Extraction protocol |
Tick gut was dissected, and RNA was harvested using Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Tick transcript data was downloaded from the VectorBase (https://vectorbase.org/vectorbase/app/) and indexed using the kallisto-index. The reads from the sequencer were pseudo-aligned with the index reference transcriptome using kallisto. The counts generated were processed by DESeq2 in RStudio (https://rstudio.com). Genome_build: IscaW1 Supplementary_files_format_and_content: tab-delimited text files include baseMean, log2Foldchange etc. for each gene
|
|
|
Submission date |
Mar 22, 2021 |
Last update date |
Dec 08, 2021 |
Contact name |
Xiaotian Tang |
E-mail(s) |
xiaotian.tang@yale.edu
|
Organization name |
Yale School of Medicine
|
Department |
Internal Medicine
|
Street address |
300 Cedar Street
|
City |
New Haven |
State/province |
CT |
ZIP/Postal code |
06519 |
Country |
USA |
|
|
Platform ID |
GPL29893 |
Series (1) |
GSE169293 |
Tick Adiponectin Receptor-Mediated Phospholipid Metabolism Contributes to Borrelia burgdorferi Colonization |
|
Relations |
BioSample |
SAMN18397197 |
SRA |
SRX10404583 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
|
|
|
|
|