|
| Status |
Public on Mar 09, 2010 |
| Title |
PAXgeneBlood_PreTreatment_No Inflammation (NI)_Verification set_rep1_NI30 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral Blood, sampling at pre treatment with infliximab infusion, no inflammation at 14 weeks after the treatment
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: whole blood
gender: female
age: 63y
serum crp level at 14 weeks (mg/dl): 0.1
|
| Treatment protocol |
Right before the infusion of Infliximab.
|
| Growth protocol |
RA patients who were resistant to standard MTX treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Blood (2.5ml) was collected from the patient into PAXgene Blood RNA Kit (QIAGEN, Valencia, CA). Total RNA was extracted according to the manufacturer’s protocol
|
| Label |
Cy3
|
| Label protocol |
Sample RNA was amplified and 250 ng of total RNA was labeled using Cy3.
|
| |
|
| Hybridization protocol |
Hybridization was performed for 17 hrs at 65°C.
|
| Scan protocol |
Arrays were scanned on an Agilent DNA microarray scanner.
|
| Description |
Gene expression of whole blood cells from Rheumatoid Arthritis patients before infliximab treatment
|
| Data processing |
Image analyses, data extraction, and background subtraction were done with the standard procedures of Agilent Feature Extraction Software Ver.9.1.
|
| |
|
| Submission date |
Mar 09, 2010 |
| Last update date |
Mar 09, 2010 |
| Contact name |
Motohiko Tanino |
| E-mail(s) |
tanino@dna-chip.co.jp
|
| Phone |
+81-45-5211
|
| Fax |
+81-45-5219
|
| Organization name |
DNA Chip Research Inc.
|
| Street address |
1-1-43, Suehirocho, Tsurumi-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE20690 |
Efficacy of anti-TNF biologic agent, infliximab, for RA patients using transcriptome analysis of white blood cells |
|
