|
| Status |
Public on Jul 18, 2022 |
| Title |
Mock rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Calu-3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Calu-3
treatment: Mock
infection: Mock
|
| Treatment protocol |
Calu-3 cells were infected with SARS-CoV-2 (MOI = 0.5) together with or without 2-D08 (100 μM) treatment for 24 h.
|
| Growth protocol |
Calu-3 cells were cultured in MEM (Gibco) with 1% NEAA (Gibco), 1% sodium pyruvate (Gibco) and 10% (vol/vol) fetal bovine serum (Gibco). Cells were either mock-infected or infected with SARS-CoV-2 (Accession number: MT123290),virus was allowed to adsorb at 37 °C for 1 hr with gentle rocking every 15 min before incubation at 37 °C for indicated time points. Cells were infected at different MOI and time points as indicated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested using Trizol reagent.
Total RNA was isolated from cells using TRIzol reagent (Invitrogen), and sequencing was performed by Sangon Biotech. Sample quality was assessed using a Bioanalyzer (Agilent 2100 Bioanalyzer). RNA-seq libraries of polyadenylated RNA were prepared using mRNA-seq V2 Library Prep Kit and sequenced on MGISEQ-2000 platform.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
A1
|
| Data processing |
bcl2fastq conversion software v2.17 used for basecalling.
Reads were aligned to the human genome (hg38) using Hisat2
The gene expression level was calculated by using featureCounts.
Fold-change (FC) and p-values were calculated with DEseq2
Genome_build: hg38
Supplementary_files_format_and_content: Csv file containing reads count.
|
| |
|
| Submission date |
Mar 30, 2021 |
| Last update date |
Jul 18, 2022 |
| Contact name |
shi jun jie |
| Organization name |
sysu
|
| Street address |
zhongshanerlu74hao
|
| City |
guangzhou |
| ZIP/Postal code |
471000 |
| Country |
China |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE171130 |
SUMOylation restrains the degradation of ACE2 through TOLLIP-mediated selective autophagy to facilitate the host susceptibility to SARS-CoV-2 infection |
|
| Relations |
| BioSample |
SAMN18537464 |
| SRA |
SRX10474847 |
