|
Status |
Public on May 21, 2021 |
Title |
ATAC-Seq 3hr rep1 |
Sample type |
SRA |
|
|
Source name |
human fibroblast + mouse ESC fusion
|
Organisms |
Homo sapiens; Mus musculus |
Characteristics |
cell type: heterokaryon treatment: no dnAP-1 (control)
|
Treatment protocol |
To induce dnAP-1 in fibroblasts, doxycycline was added at the beginning of the co-culture step at 1000 ng/ml
|
Growth protocol |
Fibroblasts are grown in 10% FBS + DMEM. ESCs are grown with 10% FBS, 10% KSR, LIF, beta-mercaptoethanol in KO DMEM
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Cells were pelleted and permeabilized in hypotonic buffer with 0.1% IGEPAL CA-630. Nuclei were treated with transposase from Illumina (Cat FC-121-1030) Fragments were amplified with NEBNext PCR master mix and Nextera indices for 8-12 cycles
|
|
|
Library strategy |
ATAC-seq |
Library source |
genomic |
Library selection |
other |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
3hr rep1
|
Data processing |
Default Illumina base calling implementation Reads were trimmed, and then mapped to concatenated human and mouse genome using Bowtie 2 reads mapping to chrM and those with MAPQ <10 were removed peaks were called with MACS 2 Genome_build: hg38/mm10 Supplementary_files_format_and_content: bigwig read pileup centered at Tn5 29bp footprint
|
|
|
Submission date |
May 05, 2021 |
Last update date |
May 21, 2021 |
Contact name |
Helen M Blau |
Organization name |
Stanford University
|
Department |
Baxter Laboratories
|
Street address |
269 Campus Dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
|
|
Platform ID |
GPL25526 |
Series (2) |
GSE120992 |
AP-1 is a temporally regulated dual gatekeeper of reprogramming to pluripotency (ATAC-Seq) |
GSE121053 |
AP-1 is a temporally regulated dual gatekeeper of reprogramming to pluripotency |
|
Relations |
BioSample |
SAMN19024203 |
SRA |
SRX10785147 |