|
| Status |
Public on Apr 19, 2011 |
| Title |
Control_6 |
| Sample type |
RNA |
| |
|
| Source name |
Lung tissue sample from lung transplant patient
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: lung
disease status: Control
diagnosis: Normal
fvc-group: Normal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
100 ng of total RNA was dephosphorylated with calf intestinal alkaline phosphatase, followed by denaturation in dimethyl sulfoxide.
|
| Label |
Cy3
|
| Label protocol |
T4 ligase was used to join cyanine 3-cytidine bisphosphate to the termini of all single-stranded RNA (including miRNA) and the labeled RNA was purified from these reactions by gel filtration on MicroBioSpin 6 columns (Bio-Rad).
|
| |
|
| Hybridization protocol |
The purified labeled miRNA probes were hybridized for 20 h at 55 °C.
|
| Scan protocol |
The arrays were washed and scanned at 5-micron resolution with a PerkinElmer ScanArray Express array scanner.
|
| Description |
Control
|
| Data processing |
The resulting images were quantified by using Agilent’s Feature Extraction software. Further, expression data was consolidated to make representative expression profile for each probe and then normalized using quantile method (MATLAB R2009b).
|
| |
|
| Submission date |
Apr 19, 2010 |
| Last update date |
Apr 19, 2011 |
| Contact name |
Ji-Hoon Cho |
| E-mail(s) |
jcho@systemsbiology.org
|
| Organization name |
Institute for Systems Biology
|
| Street address |
401 Terry Avenue North
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL8936 |
| Series (2) |
| GSE21394 |
MicroRNA expression profiles of interstitial lung disease (ILD) patients |
| GSE21411 |
Systems biology of interstitial lung diseases |
|
