|
| Status |
Public on Jun 08, 2021 |
| Title |
mock2_rep1 [hBVPs] |
| Sample type |
RNA |
| |
|
| Source name |
mock-infected primary brain vascular pericytes day 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: primary brain vascular pericytes
viral infection: mock
|
| Treatment protocol |
POWV-LI-9 was allowed to adsorb to ~80% confluent hBVP monolayers for 1 h. Following adsorption, monolayers were washed with PBS and grown in supplemented Pericyte Media with 10% FBS. RNA was extracted 1-3 dpi. Mock-infected cells were used as control.
|
| Growth protocol |
hBVPs (passage 5), derived from elutriation of dispase-dissociated normal human brain cortex tissue were purchased from Cell Biologics (H-6023), and were grown in endothelial cell basal medium-2 MV (EBM-2 MV; Lonza) supplemented with EGM-2 MV SingleQuots (Lonza) and incubation at 37°C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using RNeasy (Qiagen) following the manufacturer protocol.
|
| Label |
biotin
|
| Label protocol |
Fragmented and labeled single-stranded cDNA was generated from total RNA using the GeneChip WT PLUS Reagent Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized to GeneChip Human Clariom S Arrays (Affymetrix) using the Hydridization, Wash and Stain kit (Affymetrix).
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix). Arrays were scanned at the Stony Brook Genomics Core Facility.
|
| Description |
Gene expression data from human brain microvascular endothelial cells mock-infected 3 dpi
|
| Data processing |
Raw data was processed with the Expression Console software (Affymetrix) for background correction and normalization. Data analysis and statistical evaluations were performed with Transcriptome Analysis Console (TAC) Software (Affymetrix).
|
| |
|
| Submission date |
Jun 07, 2021 |
| Last update date |
Jun 08, 2021 |
| Contact name |
Jonas Nascimento Conde |
| E-mail(s) |
jonas.nascimentoconde@stonybrook.edu
|
| Organization name |
Stony Brook University
|
| Street address |
Life Sciences Building
|
| City |
Stony Brook |
| State/province |
NY |
| ZIP/Postal code |
11790 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (2) |
| GSE176252 |
POWV isolate from Long Island infects brain endothelial cells and pericytes. [hBVPs] |
| GSE176253 |
POWV isolate from Long Island infects brain endothelial cells and pericytes. |
|
