|
| Status |
Public on Jun 15, 2021 |
| Title |
SAMPLE 2_Normal 70 years |
| Sample type |
RNA |
| |
|
| Source name |
Normal_lamina cribrosa cells
|
| Organism |
Homo sapiens |
| Characteristics |
subject status/treatment: Normal
age (yrs): 70
cell type: human lamina cribrosa cells
|
| Treatment protocol |
For TGFb2 treatment, lamina cribrosa were serum deprived for 24 hours, prior to treatment with 5ng/mL TGFb2 or with control for 24 hours.
|
| Growth protocol |
Lamina cribrosa cells were maintained in Ham's F10 medium (Sigma Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum. The medium was changed every 2-3 days and maintained at 37 degrees C with 5% CO2 within a humidified incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA
|
| Label |
SYBR green
|
| Label protocol |
cDNA was synthesized using the HiSpec buffer and miScript II RT kit (Qiagen, Valencaia, CA, USA).miRNA profiling was performed by a SYBR green-based real-time PCR miRNA array. (MZ117; Qiagen).
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Eye cells
|
| Data processing |
The normalization and all the data analysis were performed according to the manufacturers instructions using their web-based software package: https//://dataanalysis.qiagen.com/mirna/arrayanalysis.php (accessed on June 24, 2020)
|
| |
|
| Submission date |
Jun 14, 2021 |
| Last update date |
Jun 15, 2021 |
| Contact name |
Tara Tovar-Vidales |
| E-mail(s) |
tara.tovar-vidales@unthsc.edu
|
| Phone |
8177352339
|
| Organization name |
UNTHSC
|
| Department |
Pharmacology & Neuroscience
|
| Street address |
3500 Camp Bowie
|
| City |
FORT WORTH |
| State/province |
TX |
| ZIP/Postal code |
761072725 |
| Country |
USA |
| |
|
| Platform ID |
GPL30275 |
| Series (1) |
| GSE178137 |
MiRNA Expression in Glaucomatous and TGFb2 Treated Lamina Cribrosa cells |
|
