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Status
Public on Apr 29, 2010
Title
DC.4+.Sp.ST#9
Sample type
RNA
Source name
spleen
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Liberase Blendzymes III, DNAse I digestion CD3e, CD19, GR1, NK1.1 MACS depletion
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.4+.Sp#9, spleen CD4+ dendritic cell
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
Jul 13, 2018
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE68399 Reanalyzed by
GSE74316 Reanalyzed by
GSE115450 Reanalyzed by
GSE117080
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
15.2391
10344622
145.867
10344624
370.2875
10344633
943.2419
10344637
258.069
10344653
15.5008
10344658
166.6217
10344674
10.1279
10344679
51.5619
10344707
185.9117
10344713
295.2083
10344723
154.9816
10344725
45.7471
10344741
3370.6788
10344743
30.7933
10344750
1057.2561
10344772
39.4929
10344789
227.3465
10344797
304.2009
10344799
274.3608
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM538251_EA07068_90312_MoGene_DC2.SP#9.CEL.gz
4.6 Mb
(ftp) (http) CEL
Processed data included within Sample table
