disease state: Diffuse Large B-cell Lymphoma age: 78 Sex: F cell of origin by lymph2cx: ABC status disease-free survival_0 relapse_dead_1: 0 survival time (day): 1148 tissue: DLBCL FFPE tissue
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted from DLBCL FFPE samples using a RNeasy FFPE extraction kit (QIAGEN, Hilden, Germany) after deparaffinization with Deparaffinization Solution (QIAGEN), based on the manufacturer’s instruction.
Label
n/a
Label protocol
N/A
Hybridization protocol
300 ng total RNA was hybridized to gene-specific probes at 65˚C for 18 hours and then purified and deposited onto glass cartridges using the “high sensitivity” setting on the nCounter™ Prep Station (NanoString Technologies, Seattle, WA, USA).
Scan protocol
The number of barcodes hybridized to specific RNA targets on cartridges were directly counted by the nCounter™ Digital Analyzer with 555 fields of view setting.
Data processing
Normalization of RNA loading was performed using the geometric mean of 15 housekeeping genes (ABCF1, ALAS1, EEF1G, G6PD, GAPDH, GUSB, HPRT1, OAZ1, POLR1B, POLR2A, PPIA, SDHA, TBP, TUBB, and RPL19).
