|
| Status |
Public on Mar 07, 2022 |
| Title |
Visceral_fat_streptozotocin_bezafibrate_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Visceral fat from streptozotocin/bezafibrate-treated mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6N
age: 5-6 months
gender: male
genotype: wildtype
treatment: streptozotocin/bezafibrate-treated
tissue: visceral fat
|
| Treatment protocol |
To induce type 1 diabetes, 65 mg/kg of streptozotocin (STZ) was injected i.p. into 10-16 weeks old mice on 5 consecutive days while respective controls were mock-injected with citrate buffer. BEZ treatment for 8 weeks started 2 weeks after STZ injection. BEZ (B7273, Sigma-Aldrich) was mixed to standard mouse chow in 0.25% or 0.5% (w/w) concentration (BEZ food) by the supplier (R/M-H, Ssniff, Germany); control animals received untreated placebo food (standard diet, SD food).
|
| Growth protocol |
Animals per housed under spf conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Mini Kit (Qiagen) including DNase treatment
|
| Label |
biotin
|
| Label protocol |
WT Expression Kit (Ambion) and the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol (HWS kit)
|
| Scan protocol |
According to the Affymetrix expression protocol (GeneChip Scanner 3000 7G)
|
| Description |
STZ_BEZ_49
Gene expression data from visceral fat from streptozotocin/bezafibrate-treated mice, biological replicate 5
|
| Data processing |
RMA probeset summary with standard settings of the Affymetrix Expression Console software (v.1.2) including median polish and Sketch-Quantile normalisation
|
| |
|
| Submission date |
Jul 08, 2021 |
| Last update date |
Mar 07, 2022 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE179717 |
Bezafibrate reduces the elevated hepatic fumarate level in insulin-deficient streptozotocin mice [visceral adipose tissue] |
| GSE179719 |
Bezafibrate reduces the elevated hepatic fumarate in insulin-deficient mice. |
|
