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Sample GSM5429760 Query DataSets for GSM5429760
Status Public on Mar 07, 2022
Title Skeletal_muscle_control_rep4
Sample type RNA
 
Source name Skeletal muscle from control mice
Organism Mus musculus
Characteristics strain: C57BL/6N
age: 5-6 months
gender: male
genotype: wildtype
treatment: control
tissue: skeletal muscle
Treatment protocol To induce type 1 diabetes, 65 mg/kg of streptozotocin (STZ) was injected i.p. into 10-16 weeks old mice on 5 consecutive days while respective controls were mock-injected with citrate buffer. BEZ treatment for 8 weeks started 2 weeks after STZ injection. BEZ (B7273, Sigma-Aldrich) was mixed to standard mouse chow in 0.25% or 0.5% (w/w) concentration (BEZ food) by the supplier (R/M-H, Ssniff, Germany); control animals received untreated placebo food (standard diet, SD food).
Growth protocol Animals per housed under spf conditions
Extracted molecule total RNA
Extraction protocol miRNeasy Mini Kit (Qiagen) including DNase treatment
Label biotin
Label protocol WT Expression Kit (Ambion) and the WT Terminal Labeling Kit (Affymetrix)
 
Hybridization protocol According to the Affymetrix expression protocol (HWS kit)
Scan protocol According to the Affymetrix expression protocol (GeneChip Scanner 3000 7G)
Description Ctrl_04
Gene expression data from skeletal muscle from control mice, biological replicate 4
Data processing RMA probeset summary with standard settings of the Affymetrix Expression Console software (v.1.2) including median polish and Sketch-Quantile normalisation
 
Submission date Jul 08, 2021
Last update date Mar 07, 2022
Contact name Johannes Beckers
E-mail(s) johannes.beckers@helmholtz-munich.de
Organization name Helmholtz Zentrum Muenchen
Department Institute of Experimental Genetics
Street address Ingolstaedter Landstr. 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL6246
Series (2)
GSE179718 Bezafibrate reduces the elevated hepatic fumarate level in insulin-deficient streptozotocin mice [skeletal muscle tissue]
GSE179719 Bezafibrate reduces the elevated hepatic fumarate in insulin-deficient mice.

Data table header descriptions
ID_REF
VALUE RMA signal intensity (log2)

Data table
ID_REF VALUE
10338001 11.91214
10338002 6.351782
10338003 10.15551
10338004 9.093576
10338005 3.229024
10338006 3.357134
10338007 3.568769
10338008 4.021658
10338009 8.515703
10338010 3.23955
10338011 5.605905
10338012 3.286545
10338013 3.131764
10338014 3.192037
10338015 3.151006
10338016 7.662468
10338017 12.97129
10338018 6.673814
10338019 5.151135
10338020 8.236178

Total number of rows: 35556

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM5429760_SD_04.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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