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Status |
Public on Mar 07, 2022 |
Title |
Skeletal_muscle_streptozotocin_rep4 |
Sample type |
RNA |
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Source name |
Skeletal muscle from streptozotocin-treated mice
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Organism |
Mus musculus |
Characteristics |
strain: C57BL/6N age: 5-6 months gender: male genotype: wildtype treatment: streptozotocin-treated tissue: skeletal muscle
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Treatment protocol |
To induce type 1 diabetes, 65 mg/kg of streptozotocin (STZ) was injected i.p. into 10-16 weeks old mice on 5 consecutive days while respective controls were mock-injected with citrate buffer. BEZ treatment for 8 weeks started 2 weeks after STZ injection. BEZ (B7273, Sigma-Aldrich) was mixed to standard mouse chow in 0.25% or 0.5% (w/w) concentration (BEZ food) by the supplier (R/M-H, Ssniff, Germany); control animals received untreated placebo food (standard diet, SD food).
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Growth protocol |
Animals per housed under spf conditions
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Extracted molecule |
total RNA |
Extraction protocol |
miRNeasy Mini Kit (Qiagen) including DNase treatment
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Label |
biotin
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Label protocol |
WT Expression Kit (Ambion) and the WT Terminal Labeling Kit (Affymetrix)
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|
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Hybridization protocol |
According to the Affymetrix expression protocol (HWS kit)
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Scan protocol |
According to the Affymetrix expression protocol (GeneChip Scanner 3000 7G)
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Description |
STZ_40 Gene expression data from skeletal muscle from streptozotocin-treated mice, biological replicate 4
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Data processing |
RMA probeset summary with standard settings of the Affymetrix Expression Console software (v.1.2) including median polish and Sketch-Quantile normalisation
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Submission date |
Jul 08, 2021 |
Last update date |
Mar 07, 2022 |
Contact name |
Johannes Beckers |
E-mail(s) |
johannes.beckers@helmholtz-munich.de
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Organization name |
Helmholtz Zentrum Muenchen
|
Department |
Institute of Experimental Genetics
|
Street address |
Ingolstaedter Landstr. 1
|
City |
Neuherberg |
ZIP/Postal code |
85764 |
Country |
Germany |
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Platform ID |
GPL6246 |
Series (2) |
GSE179718 |
Bezafibrate reduces the elevated hepatic fumarate level in insulin-deficient streptozotocin mice [skeletal muscle tissue] |
GSE179719 |
Bezafibrate reduces the elevated hepatic fumarate in insulin-deficient mice. |
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