|
| Status |
Public on Jan 11, 2022 |
| Title |
M-CSF donor 2 |
| Sample type |
RNA |
| |
|
| Source name |
blood
|
| Organism |
Homo sapiens |
| Characteristics |
stimulation: LPS-stimulated
|
| Treatment protocol |
On day 7, cells were activated by 10 ng/ml lipopolysaccharide (LPS).
|
| Growth protocol |
Human monocytes were isolated from the blood using Milteny negative separation kit. Monocytes were differentiated into macrophages and dendritic cells for 7 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol and further precipitated using ammonium acetate.
|
| Label |
biotin
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina protocol (Illumina Hybridiation Oven Model 5521)
|
| Scan protocol |
Illumina iScan scanner
|
| Description |
M-CSF
donor 2
|
| Data processing |
The data were background corrected and quantile normalized using the neqc function from the limma R package (v3.46.0).
|
| |
|
| Submission date |
Jul 14, 2021 |
| Last update date |
Jan 11, 2022 |
| Contact name |
Manuela Rossol |
| E-mail(s) |
manuela.rossol@medizin.uni-leipzig.de
|
| Organization name |
University of Leipzig
|
| Lab |
Experimental Rheumatology
|
| Street address |
Liebigstr. 19
|
| City |
Leipzig |
| ZIP/Postal code |
04103 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE180111 |
Danger signal initiates differentiation of monocytes into osteopontin‑producing macrophages II |
|
