|
| Status |
Public on Nov 03, 2021 |
| Title |
C3 |
| Sample type |
RNA |
| |
|
| Source name |
healthy woman with mamoplasty
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: normal breast tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from all normal breast tissues used in this study was obtained using the RNeasy Mini Kit (Qiagen, CA, USA) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
The biotinylated cRNA was subsequently hybridized to a GeneChip Human Exon 1.0 ST Array (Affymetrix, CA, USA).
|
| Scan protocol |
The GeneChip arrays were washed and stained according to the manufacturer’s protocols and scanned using a GeneChip® Scanner 3000 (Affymetrix)
|
| Description |
The normal breast tissues were obtained from healthy women that performed mamoplasty at Hospital Universitário de Londrina (Londrina, Paraná, Brazil)
|
| Data processing |
Affymetrix Expression Console Software version 1.0 was used to create summarized expression values (CHP-files). The Robust Multichip Analysis (RMA) algorithm was applied. The data were analyzed with Partek® software (http://www.partek.com).
|
| |
|
| Submission date |
Jul 26, 2021 |
| Last update date |
Nov 03, 2021 |
| Contact name |
Renata Binato |
| E-mail(s) |
renata.binato@inca.gov.br
|
| Phone |
+55(21)32071874
|
| Organization name |
INCA
|
| Lab |
Lab. Célula Tronco
|
| Street address |
Praça Cruz Vermelha 23/ 6 andar ala C
|
| City |
Rio de Janeiro |
| State/province |
Rio de Janeiro |
| ZIP/Postal code |
20230-130 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL5175 |
| Series (1) |
| GSE180835 |
Expression profile of Normal Breast Tissue in Brazilian women |
|
