|
| Status |
Public on Jan 10, 2011 |
| Title |
Normal-ERα-positive breast cancer 68 |
| Sample type |
RNA |
| |
|
| Source name |
ERα-positive breast tumors without PIK3CA mutation
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: primary unilateral non-metastatic postmenopausal ERα-positive breast tumor
gender: woman
age: 74 years
|
| Biomaterial provider |
breast tumors were excised from women at the René Huguenin Hospital (Saint-Cloud, France) from 1978 to 2008
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from breast tissue samples by using acid-phenol guanidium method.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
|
| |
|
| Hybridization protocol |
Gene chips were hybridized using standard Affymetrix protocol.
|
| Scan protocol |
Gene chips were scanned using standard Affymetrix protocol.
|
| Description |
Gene expression data from ERα-positive breast tumor without PIK3CA mutation
|
| Data processing |
BRB Array tools (version 3.6.0 available on http://linus.nci.nih.gov/BRB-ArrayTools.html) were used to import CEL files applying the Robust Method Average (RMA) normalization and to analyze expression genes.
|
| |
|
| Submission date |
May 27, 2010 |
| Last update date |
Jan 10, 2011 |
| Contact name |
clairac geraldine |
| E-mail(s) |
clairac@crh1.org
|
| Phone |
33147112310
|
| Fax |
33147111696
|
| Organization name |
Centre Rene Huguenin
|
| Department |
Oncogenetique
|
| Lab |
INSERM U735
|
| Street address |
35 rue Daily
|
| City |
SAINT-CLOUD |
| ZIP/Postal code |
92210 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE22035 |
Gene expression data in estrogen receptor alpha positive breast tumors with and without PIK3CA mutations. |
|
