|
| Status |
Public on Mar 05, 2012 |
| Title |
DGM-01505 [gene_expr] |
| Sample type |
RNA |
| |
|
| Source name |
small airway epithelium from healthy nonsmokers or healthy smokers
|
| Organism |
Homo sapiens |
| Characteristics |
department of genetic medicine id: DGM-01505
smoking status: NS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
n/a
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 03, 2010 |
| Last update date |
Mar 05, 2012 |
| Contact name |
Yael Strulovici-Barel |
| E-mail(s) |
yas2003@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Department of Genetic Medicine
|
| Lab |
Crystal
|
| Street address |
1300 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (10)
|
| GSE22047 |
Modulation of Cystatin A Expression in Human Airway Epithelium Related to Genotype, Smoking COPD and Lung Cancer |
| GSE27681 |
RNA-Seq Quantification of the Transcriptome of Genes Expressed in the Small Airway Epithelium of Nonsmokers and Smokers |
| GSE30063 |
Epithelial Expression of Toll-like Receptor 5 is Modulated in Healthy Smokers and Smokers with Chronic Obstructive Lung Disease |
| GSE34450 |
Genes Associated with MUC5AC Expression in the Human Airway Epithelium |
| GSE63127 |
Cigarette smoking induces changes in airway epithelial expression of genes associated with monogenic lung disorders |
| GSE64614 |
Distal-to-Proximal Re-patterning of Small Airway Epithelium in Smoking-associated Chronic Obstructive Pulmonary Disease |
| GSE76324 |
Role of OSGIN1 in Mediating Smoking-induced Autophagy in the Human Airway Epithelium [array] |
| GSE76327 |
Role of OSGIN1 in Mediating Smoking-induced Autophagy in the Human Airway Epithelium |
| GSE77658 |
POU2AF1 Functions in the Human Airway Epithelium to Regulate Expression of Host Defense Genes [array] |
| GSE77659 |
POU2AF1 Functions in the Human Airway Epithelium to Regulate Expression of Host Defense Genes |
|
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